The µ-Slide 2 well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when
normal phase contrast microscopy is being used. Opposed to the classic µSlide 2 well, the Ph+ version provides a
special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast
effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of
liquids.This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.
Cross Section Through a Well of the µ-Slide 2 well Ph+ with a Transmitted Light Beam Path
The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface,
leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.Working with
the µ-Slide 2 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast
is due to the parallel beam path that is created by the plate.
